Coding

Part:BBa_K1903017

Designed by: Rafael Montenegro Marín   Group: iGEM16_TEC-Costa_Rica   (2016-10-17)


dCas9 B coding sequence

The sequence is a coding part for a dCAs9 protein assembly with golden gate and has two windows frames for protein insertions at amino acids: N588 and N888.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1046
    Illegal BglII site found at 2822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3536
    Illegal SapI.rc site found at 1159


[edit]
Categories
Parameters
None